酵母裂解酶Zymolyase? 是通過藤黃節(jié)桿菌深層培養(yǎng)獲得的酶制劑。它能有效的裂解活酵母細胞細胞壁，產(chǎn)生原生質(zhì)體或原生質(zhì)球。其中主要負責(zé)裂解活酵母細胞的酶成分是?-1,3- 葡聚糖昆布五糖水解酶，通過對?-1,3- 葡聚糖連接位水解葡萄糖聚合物。我們提供兩種包裝Zymolyase-20T 和Zymolyase-100T。Zymolyase-20T 經(jīng)過硫酸銨沉淀，Zymolyase-100T 經(jīng)過親和色譜法進一步純化制備。裂解活性取決于酵母菌株、酵母生長階段或培養(yǎng)條件(6-8)。更多信息下方參考文獻提供(12-16)。
[ 反應(yīng)混合物] 酶溶液: 1ml(0.05-0.1mg/mlforZymolyase?-20T) (0.012-0.024mg/mlforZymolyase?-100T) 啤酒酵母細胞懸液: 3ml (2 mg/ml) 1/15M 磷酸鹽緩沖液 :5 ml (pH7.5) 蒸餾水:1 ml
參考文獻
1. Kaneko, T., Kitamura, K and Yamamoto, Y.: J. Gen. Appl. Microbiol., 15, 317 (1969) 2. Kitamura, K., Kaneko, T. and Yamamoto, Y.: Arch. Biochem. Biophys., 145, 402 (1971) 3. Kitamura, K., Kaneko, T. and Yamamoto, Y.: J. Hen. Appl. Microbiol., 18, 57 (1972) 4. Kitamura, K. and Yamamoto, Y.: Arch. Biochem. Biophys., 153, 403 (1972) 5. Kaneko, T., Kitamura, K. and Yamamoto, Y.: Agric. Biol. Chem., 37, 2295 (1973) 6. Kitamura, K., Kaneko, T. and Yamamoto, Y.: J. Gen Appl. Microbiol., 20, 323 (1974) 7. Kitamura, K. and Yamamoto,.: Agric. Biol. Chem., 45, 1761 (1981) 8. Katamura, K. and Tanabe, K.: Agric, Biol. Chem., 46, 553 (1982) 9. Katamura, K.: J. Ferment. Technol., 60, 257 (1982) 10. Kitamura, K.: Agric. Biol. Chem., 46, 963 (1982) 11. Kitamura, K.: Agric. Biol. Chem., 46, 2093 (1982) 12. Calza R. E., Schroeder A. L.: J. Gen. Microbiol., 129, 413 (1983) 13. Iizuka Masaru, Torii Yasuhiko, Yamamoto Takehiko: Agric. Biol. Chem., 47 (12), 2267 (1983) 14. Shibata Nobuyuki, Kobayashi Hidemitsu, tojo Menehiro, Suzuki Shigeo: Arch. Biochem. Biophys., 251(2), 697 (1986) 15. Iijima Y., Yanagi S. O.: Agric. Biol. Chem., 50 (7), 1855 (1986) 16. Herrero Enrique, Sanz Pascual. Sentandreu Rafael: J. Gen. Microbiol., 133 (10), 2895 (1987
